GSM7000659: Sphaeroforma_arctica_1; Sphaeroforma arctica; OTHER - SRA

SRX19205915: GSM7000659: Sphaeroforma_arctica_1; Sphaeroforma arctica; OTHER
1 ILLUMINA (NextSeq 500) run: 18.6M spots, 5.6G bases, 2.4Gb downloads

External Id: GSM7000659_r1

Submitted by: Danko and Lis labs, Molecular Biology and Genetics, Cornell University

Study: Pol II pausing is a milestone on the road to complex animals

PRJNA929029 • SRP419683 • All experiments • All runs

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PRO-seq experiments in multiple organisms. Also, PRO-seq experiment of Nelfb and Nelfe degron mESC in serum/LIF with or without dTAG-13. Overall design: We used Precision Run-On and Sequencing (PRO-seq) to study transcription in 20 extant organisms that represent very diverse branches on the tree of life, including multiple species near the base of the Metazoa phylogeny and the transition between plants and animals. We also used PRO-seq to study mESCs after degrading NELF-B or NELF-E.

Sample: Sphaeroforma_arctica_1

SAMN32949468 • SRS16614675 • All experiments • All runs

Organism: Sphaeroforma arctica

Library:

Name: GSM7000659

Instrument: NextSeq 500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Construction protocol: RNA extraction, run-on, and library preparation were executed according to “Mahat, D. B. et al. Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). Nat. Protoc. 11, 1455–1476 (2016).” (PMID: 27442863). Library prep was performed using Illumina custom adapters: Reverse 3' RNA adapter (/5Phos/NNNNNNGAUCGUCGGACUGUAGAACUCUGAAC/3InvdT) and Reverse 5' RNA Adapter (5'-CCUUGGCACCCGAGAAUUCCA-3').

Runs: 1 run, 18.6M spots, 5.6G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR23260647	18,568,129	5.6G	2.4Gb	2024-02-05

ID:: 26407910

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